I've been using the same bottle of Prozinc since March 8th, and haven't had any problems with its effectiveness. Motska is on such a small dose (.3 to .4 u twice per day) that we're only 1/2 way through the bottle. As long as it's sterile (the needle doesn't ever touch anything else before inserting into the vial), then in theory, you should still be fine for quite a while.
I will say this, though.... When it does come time to start a new vial, I'll be doing it on a day when I am at home so that I can keep an eye on her and test her every few hours -- just in case.