I've been using the same pen since the middle of August and it has been just as effective. I still have another 3 weeks or so left out of it. In fact, I got her pen refilled today at the pharmacy and the pharmacist even said a pen will last 6-8 weeks if kept in the fridge. He said what I already was told be the vet...that the pharmacists say you can't keep it longer than up to 2 months and the vets say you definitely can keep it for about 5 or so. The pharmacist also told me that the way you know if your insulin is no longer as effective/potent due to age is if you begin seeing your cat's BG numbers rise. Hey, just look at Olive's SS. I've been using this same pen all this time and her BG is without a doubt DOWN. Proof is in the pudding as they say. You definitely don't have to throw out your insulin after only 28 days.