Yeah, typically you won't get much action when the dose is reduced that much, I wouldn't be too worried, but still a good idea to get an early cycle spot test in to be sure. You shot 1u though on a blue PS before and got good numbers, so my expectation on .25 would be that you will just see higher numbers.
When your PMPS is lower than the mid-cycle like today, it can be a sign the dose is too high, so you may want to reduce a little in the morning, maybe go back to 1u, or you could try something like a fat 1.
Hope her poopy problems are resolved soon. Do you think it's from the canned food? I know it took us a number of tries to find an LC food that agreed with Bix. Once we found the right one though, he did a lot better than he had on dry.
If the fortiflora and metro don't fix things and you've already tried other foods and stuff, I would ask about giving Budesonide a try. It helped Bix a lot with vomiting, and affects their BGs a lot less than something like Pred (should the vet suggest that).