Hi RockStar!
I use the exact same glucometer with the lancet that came with the kit (I believe they call it Microlet 2).
I couldn't get blood from between the vein and edge of ear so i pricked her vein
The ear is probably not warm enough and/or you have not used something "firm enough" to really put a pressure on your cat's ear when using the pen.
I dont use a flashlight anymore, but during the first couple of days I was using a SMALL flashlight to see though my cat's ear while it also served as a "firm surface" to put the slightly moist cotton wool on. If you read the instruction for humans, you'll see how they insist on really pressing the pen on the skin, it also applies for cats. Make sure you select the fifth depth (so the deepest) setting (illustrated by 5 different drop of blood sizes on the lancet). Warm up your cat's ear with a sock full of rice or anything else. You'll see the huge difference of bleeding between a cold and warm ear, and you can see it even more clearly with a flashlight: if the main vein looks red and its size increased and all the little veins are visible, it means your cat's ear is filled up with blood and ready for a test! If the main vein is pink or dark pink but not quite red and you cant see all the tiny vessels joining that main vein, it means the ear wont bleed easily cuz it's still too cold.
With a cotton wool slightly moist or anything firm enough, but yet a little flexible to avoid hurting your cat, you really have to apply pressure on the lancet.
Otherwise if it's easier for you to do it manually, why not!
So the reading was 16.4 mmol/L - can someone help me understand this? When i first took her to the vet, their machine showed 27. but since this is a human tester, is there a difference? how do i tell? or do i just read it as is and consider this a bit high? i know i have to do more tests to see the fluctuation.
Did your cat also receive an insulin injection after the reading at the vet? Human testers, and especially this one is supposed to work fine on either humans or animals. If you REALLY want to make sure, you could go to the vet with your own glucometer, ask him to take a blood sample and both of you test that sample and compare readings. I did that myself when I got the Bayer contour and the difference was like 0.2 or something, so really nothing to worry about.
:?: What insulin are you giving to your cat? and how many units?
You mentioned that the reading you got at home (16.4) was a +1, which means one hour after an insulin shot right? If so then dont panic, 16.4 is still a high result... and you dont want to keep your cat at 27 for a long period of time.